• DSpace Universidad Indoamerica
  • Publicaciones Científicas
  • Artículos Científicos Indexados
  • Please use this identifier to cite or link to this item: https://repositorio.uti.edu.ec//handle/123456789/3007
    Title: Direct Color Observation of Light-Driven Molecular Conformation-Induced Stress
    Authors: Pujol-Vila, Ferran
    Escudero, Pedro
    Guell-Grau, Pau
    Pascual-Izarra, Carlos
    Villa, Rosa
    Álvarez, Mar
    Issue Date: 2022
    Publisher: Small Methods. Volume 6, Issue 2
    Abstract: Although usually complex to handle, nanomechanical sensors are exceptional, label-free tools for monitoring molecular conformational changes, which makes them of paramount importance in understanding biomolecular interactions. Herein, a simple and inexpensive mechanical imaging approach based on low-stiffness cantilevers with structural coloration (mechanochromic cantilevers (MMC)) is demonstrated, able to monitor and quantify molecular conformational changes with similar sensitivity to the classical optical beam detection method of cantilever-based sensors (≈4.6 × 10–3 N m–1). This high sensitivity is achieved by using a white light and an RGB camera working in the reflection configuration. The sensor performance is demonstrated by monitoring the UV-light induced reversible conformational changes of azobenzene molecules coating. The trans-cis isomerization of the azobenzene molecules induces a deflection of the cantilevers modifying their diffracted color, which returns to the initial state by cis-trans relaxation. Interestingly, the mechanical imaging enables a simultaneous 2D mapping of the response thus enhancing the spatial resolution of the measurements. A tight correlation is found between the color output and the cantilever's deflection and curvature angle (sensitivities of 5 × 10–3 Hue µm–1 and 1.5 × 10–1 Hue (°)–1). These findings highlight the suitability of low-stiffness MMC as an enabling technology for monitoring molecular changes with unprecedented simplicity, high-throughput capability, and functionalities.
    URI: https://onlinelibrary.wiley.com/doi/abs/10.1002/smtd.202101283
    Appears in Collections:Artículos Científicos Indexados

    Files in This Item:
    There are no files associated with this item.

    This item is licensed under a Creative Commons License Creative Commons